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9D9
9D9
規格:
價格:詢價
編號:B163856
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
9D9
商品貨號
B163856
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with LDL receptors of normal rabbits but not with those of Watanabe Heritable Hyperlipidemic rabbits.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Antigen Expression
H-2d
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine low density lipoprotein (LDL) receptor,H-2d
Cellular Products
immunoglobulin; monoclonal antibody; against bovine low density lipoprotein (LDL) receptor
Comments
Mice were immunized with trypsin digested bovine adrenal LDL receptor.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with LDL receptors of normal rabbits but not with those of Watanabe Heritable Hyperlipidemic rabbits.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Maintain cell concentration between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
JL Goldstein
Deposited As
mouse (B cell); mouse (myeloma)
References

Huettinger M, et al. Use of monoclonal anti-receptor antibodies to probe the expression of the low density lipoprotein receptor in tissues of normal and Watanabe Heritable Hyperlipidemic rabbits. J. Clin. Invest. 74: 1017-1026, 1984. PubMed: 6088578

Kita T, et al. Deficiency of low density lipoprotein receptors in liver and adrenal gland of the WHHL rabbit, an animal model of familial hypercholesterolemia. Proc. Natl. Acad. Sci. USA 78: 2268-2272, 1981. PubMed: 6264472

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