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A431NS

規格:

價格:詢價

編號:B163883

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

A431NS

商品貨號

B163883

Organism

Homo sapiens, human

Tissue

skin/epidermis

培養基

Product Format

frozen

Morphology

epithelial

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

epidermoid carcinoma

Age

85 years

Gender

female

Applications

While cell sensitivity to drugs tend to be changeable, the A431NS cell line has a stable sensitivity to LIC. The cell line can be used to test the antiproliferation activity of LIC.

Storage Conditions

liquid nitrogen vapor temperture

Derivation

A431NS was derived from the A431 cell line (ATCC CRL-1555) in 1997 by repeated subculturing to select cells that detached from the substrate easily.

Clinical Data

female

85 years

Comments

The polyinosinic-polycytidylic acid/cationic liposome complex (LIC) inhibits cancer cell growth by the induction of apoptosis.

LIC has strong cytotoxic effects on the A431NS cell line but neither polyinosinic-polycytidylic acid alone nor cationic liposome alone have antiproliferation effects.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes used in this protocol are for 75 cm² flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.
Briefly rinse the cell layer with PBS to remove all traces of serum which contains trypsin inhibitor.
Remove the PBS completely and add 2.0 to 3.0 ml of 0.25% (w/v) Trypsin-053mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 mL of complete growth medium containing 10% FBS and aspirate cells by gently pipetting.
To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes.
Discard supernatant and resuspend cells in fresh culture medium. Add appropriate aliquots of cell suspension to new culture vessels.
Place culture vessels in incubators at 37°C.

Subcultivation Ratio: 1:3 to 1:6.

Medium Renewal: Every 2 to 3 days.

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperture

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 11,12

D13S317: 9,13

D16S539: 12,14

D5S818: 12,13

D7S820: 10

THO1: 9

TPOX: 11

vWA: 15,17

Name of Depositor

K Hirabayashi

Deposited As

human

References

Hirabayashi K, et al. Inhibition of cancer cell growth by polyinosinic-polycytidylic acid/cationic liposome complex: a new biological activity. Cancer Res. 59: 4325-4333, 1999. PubMed: 10485480

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