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A-704
A-704
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B163890
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
A-704
商品貨號(hào)
B163890
Organism
Homo sapiens, human
Tissue
kidney
培養(yǎng)基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
adenocarcinoma
Age
78 years
Gender
male
Applications
This cell line is a suitable transfection host. ref
Storage Conditions
liquid nitrogen vapor phase
Karyotype
(P59) diploid to hyperdiploid, hypertriploid to hypertetraploid with abnormalities including breaks, dicentrics and endoreduplication
Derivation
The A-704 line was derived by D.J. Giard as indicated in the description for ATCC HTB-41.
Clinical Data
male
Tumorigenic
No
Effects
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 7,8
D13S317: 8
D16S539: 12,13
D5S818: 10,11
D7S820: 10
THO1: 7,9
TPOX: 11
vWA: 14,18
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1-2
Name of Depositor
DJ Giard
Deposited As
Homo sapiens
References

Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758

Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

Cross References

Nucleotide (GenBank) : U02327 Human clone ndf19 neu differentiation factor mRNA, partial cds.

Nucleotide (GenBank) : U02330 Human clone ndf33 neu differentiation factor mRNA, partial cds.

Nucleotide (GenBank) : U02326 Human clone ndf43 neu differentiation factor mRNA, complete cds.

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