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Ab 23.1
Ab 23.1
規格:
價格:詢價
編號:B163900
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Ab 23.1
商品貨號
B163900
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Carcinoma
Applications
Animals were immunized with membrane preparations of NIH3T3 cells engineered to overproduce erbB protein.
The antibody reacts with the extracellular domain of gp185erbB-2.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X8g8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human erbB-2 protein
Cellular Products
immunoglobulin; monoclonal antibody; against human erbB-2 protein
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with membrane preparations of NIH3T3 cells engineered to overproduce erbB protein.
Spleen cells were fused with P3X8g8.653 myeloma cells.
The antibody reacts with the extracellular domain of gp185erbB-2.
In vitro and in vivo the antibody has no effect upon N87 cells (an erbB overexpressing human gastric carcinoma).
However, in combination with Ab 21.1 (see ATCC HB-11601) in vitro growth of N87 is suppressed and in vivo N87 xenografts can prevented or be caused to regress by a combination of the two antibodies.
The antibodies induce autophosphoryaltion of the gp185erbB-2 protein.
Complete Growth Medium
Depositor's culture medium: Dulbecco's modified Eagle's medium, 10% NCTC 109,
15% fetal bovine serum, 1% hybridoma cloning media.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG1
Name of Depositor
Aronex Pharmaceuticals Inc.
U.S. Patent Number
References

King CR, et al. Anti-erbB-2 antibodies, combinations thereof, and therapeutic and diagnostic uses thereof. US Patent 5,587,458 dated Dec 24 1996

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