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AE-1
AE-1
規格:
價格:詢價
編號:B163909
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
AE-1
商品貨號
B163909
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody binds to human and monkey acetylcholinesterase, but reacts with a different epitope than AE-2 (ATCC HB-73).
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions
liquid nitrogen vapor phase
Derivation
Animals were immunized with purified human erythrocyte acetylcholinesterase.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Cellular Products
immunoglobulin; monoclonal antibody; against human acetylcholinesterase
Comments
Animals were immunized with purified human erythrocyte acetylcholinesterase.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds to human and monkey acetylcholinesterase, but reacts with a different epitope than AE-2 (ATCC HB-73).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension. An inoculum of 2 X 10(5) viable cells/ml is recommended.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Isotype
IgG1
Antigenic Determinant
Acetylcholin esterase, human
Name of Depositor
DM Fambrough
Deposited As
mouse (B cell); mouse (myeloma)
References

Fambrough DM, et al. Acetylcholinesterase of human erythrocytes and neuromuscular junctions: homologies revealed by monoclonal antibodies. Proc. Natl. Acad. Sci. USA 79: 1078-1082, 1982. PubMed: 6175961

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