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AHH-1
AHH-1
規格:
價格:詢價
編號:B163921
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
AHH-1
商品貨號
B163921
Organism
Homo sapiens, human
Tissue
peripheral blood
培養基
Cell Type
B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2  [Cells contain Herpesvirus]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
33 years
Gender
male
Ethnicity
Caucasian
Applications
The cells have a high level of oxidative activity, and and can be used in mutagenesis assays for substances that are metabolized into mutagenic products.
They are are sensitive to killing by benzo[a]pyrene.
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Karyotype
near diploid
Images
ATCC CRL-8146 Cell Micrograph
Clinical Data
male
Caucasian
33 years
Comments
The cells have a high level of oxidative activity, and and can be used in mutagenesis assays for substances that are metabolized into mutagenic products. They are are sensitive to killing by benzo[a]pyrene. The cells carry an endogenous Epstein-Barr virus (EBV) genome and are EBNA positive.
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; horse serum, 10%
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: 2 to 3 times per week
Note: The cells grow as floating aggregates of large cell clusters.
Cryopreservation
Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 10
D5S818: 12,13
D7S820: 10,11
THO1: 6,9.3
TPOX: 8,9
vWA: 18,19
Population Doubling Time
16 to 19 hrs
Name of Depositor
Massachusetts Institute of Technology
Deposited As
Homo sapiens
U.S. Patent Number
References

Crespi CL, Thilly WG. Mutation assays involving blood cells that metabolize toxic substances. US Patent 4,532,204 dated Jul 30 1985

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