Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The cells grow in suspension and subcultures are prepared by diluting 0.5 mL of cell suspension with 4.5 mL of fresh culture medium and dispensing into a new flask. Culture at 23°C to 25°C.
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:12 is recommended
Medium Renewal: At the time of subcultivation (approximately every 6 days)
. . Nature 195: 1, 1962.
Yunker CE, et al. Adaptation of an insect cell line (Grace's Antheraea cells) to medium free of insect hemolymph. Science 155: 1565-1566, 1967. PubMed: 6020481
Yunker CE, Cory J. Infection of Grace's Antheraea cells with Arboviruses. Am. J. Trop. Med. Hyg. 17: 889-893, 1968. PubMed: 5726135
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
<dfn id="7afvf"></dfn>
<dfn id="7afvf"></dfn>