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AS 33

規(guī)格:

價(jià)格:詢(xún)價(jià)

編號(hào):B163958

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱(chēng)

AS 33

商品貨號(hào)

B163958

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養(yǎng)基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Carcinoma

Strain

Strain:? BALB/c (B cell); BALB/c (myeloma)

Applications

The antibody reacts with some colon and pancreatic carcinoma cell lines as well as some T cell leukemia lines.

Some reactivity was also detected in salivary gland and breast tissue.

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with NS-1 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against a heat stable human carcinoma associated antigen

Cellular Products

immunoglobulin; monoclonal antibody; against a heat stable human carcinoma associated antigen

Tumorigenic

Yes

Effects

Yes, forms ascites in nude mice

Comments

Animals were immunized with the ASPC-1 human pancreatic carcinoma cell line.

Spleen cells were fused with NS-1 myeloma cells.

The antibody reacts with some colon and pancreatic carcinoma cell lines as well as some T cell leukemia lines.

In tissues, the reactivity is restricted to carcinomas and normal colonic mucosa.

Some reactivity was also detected in salivary gland and breast tissue.

Other normal tissues test were negative.

The AS 33 antigen is heat stable.

Complete Growth Medium

RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%

Subculturing

Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Isotype

IgG2a , IgG2a

Name of Depositor

Sloan-Kettering Institute for Cancer Research

Deposited As

mouse (B cell); mouse (myeloma)