美女考逼,非洲黄色片,欧美不卡一区二区三区

B38.1

規(guī)格:

價(jià)格:詢價(jià)

編號(hào):B163983

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

加入購(gòu)物車(chē)

在線咨詢收藏

產(chǎn)品名稱

B38.1

商品貨號(hào)

B163983

Organism

Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)

Cell Type

hybridoma: B lymphocyte

培養(yǎng)基

Product Format

frozen

Morphology

lymphoblast

Culture Properties

suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

Carcinoma,sarcoma

Applications

The antibody reacts weakly with extracts of both metastases and with live cells, and strongly with live BT-20 (ATCC HTB-19), MCF7 (ATCC HTB-22) and or ZR-75-1 (ATCC CRL-1500) mammary carcinoma cells.

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Derivation

Spleen cells were fused with NS-1 myeloma cells.

Genes Expressed

immunoglobulin; monoclonal antibody; against human mammary tumor cells

Cellular Products

immunoglobulin; monoclonal antibody; against human mammary tumor cells

Comments

Animals were immunized with membrane enriched fractions of extracts from either of two human breast tumor metastases to the liver (Met 1 and Met 2).

Spleen cells were fused with NS-1 myeloma cells.

The antibody reacts weakly with extracts of both metastases and with live cells, and strongly with live BT-20 (ATCC HTB-19), MCF7 (ATCC HTB-22) and or ZR-75-1 (ATCC CRL-1500) mammary carcinoma cells.

The antibody does not react with liver extracts or a variety of live normal and sarcoma cells.

Complete Growth Medium

Culture Medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum,
20%.

Subculturing

Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended

Medium Renewal: 3 times per week

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Cryopreservation

culture medium, 92.5%; DMSO, 7.5%

Isotype

IgG1

Name of Depositor

NIH

Deposited As

mouse (B cell); mouse (myeloma)

References

Colcher D, et al. A spectrum of monoclonal antibodies reactive with human mammary tumor cells. Proc. Natl. Acad. Sci. USA 78: 3199-3203, 1981. PubMed: 6789331

Colcher D, et al. Process for producing monoclonal antibodies reactive with human breast cancer. US Patent 4,522,918 dated Jun 11 1985

王經(jīng)理

手機(jī)號(hào)：17280875617

QQ：1438578920
胡經(jīng)理

手機(jī)號(hào)：13345964880

QQ：2438244627
周經(jīng)理

手機(jī)號(hào)：17757487661

QQ：1296385441
于經(jīng)理

手機(jī)號(hào)：18067160830

QQ：2088210172
沈經(jīng)理

手機(jī)號(hào)：19548299266

QQ：2662369050
李經(jīng)理

手機(jī)號(hào)：13626845108

QQ：972239479
曹經(jīng)理

手機(jī)號(hào)：18662422060

QQ：3671869992