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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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BALB/B 0.75BAE A.1R.1 HD A.8
BALB/B 0.75BAE A.1R.1 HD A.8
規格:
價格:詢價
編號:B163993
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
BALB/B 0.75BAE A.1R.1 HD A.8
商品貨號
B163993
Organism
Mus musculus, mouse
Cell Type
fibroblastchemically transformed
培養基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
normal
Age
embryo
Strain
BALB.B
Applications
This line was derived from the BALB/B CL.6 normal embryonic fibroblast cell line by transformation with benzanthracene epoxide.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions
liquid nitrogen vapor phase
Derivation
This line was derived from the BALB/B CL.6 normal embryonic fibroblast cell line by transformation with benzanthracene epoxide.
Tumorigenic
No
Effects
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments
This line was derived from the BALB/B CL.6 normal embryonic fibroblast cell line by transformation with benzanthracene epoxide.
The cells will grow in 0.3% agarose.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor
P Patek, J Collins, M Cohn
Deposited As
Mus musculus
References

Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934

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