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BALB/c 10CrMCA A.2R.1

規格:

貨期:

編號:B163994

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

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產品名稱

BALB/c 10CrMCA A.2R.1

商品貨號

B163994

Organism

Mus musculus, mouse

Cell Type

fibroblast chemically transformed

Product Format

frozen

Morphology

fibroblast

Culture Properties

adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

normal

Age

embryo

Strain

BALB/c

Derivation

This line was derived from BALB/c CL.7 (ATCC TIB-80) by transformation with crystalline methylcholanthrene.

Tumorigenic

Yes

Effects

Yes, in ATxFL mice but not in normal BALB/c mice

Comments

The cells will grow in 0.3% agarose.

Tested and found negative for ectromelia virus (mousepox).

Complete Growth Medium

The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Remove old medium, add fresh 0.25% trypsin for 1 minute, remove trypsin and let the culture sit at room temperature until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Name of Depositor

P Patek, J Collins, M Cohn

Deposited As

Mus musculus

References

Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934

B163997：BALB/c CL.7

B163996：BALB/c AMuLV A.6R.1

B163995：BALB/c AMuLV A.3R.1

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