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BBM.1
BBM.1
規(guī)格:
價(jià)格:詢價(jià)
編號:B164006
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
BBM.1
商品貨號
B164006
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with P3X63Ag8 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human Beta-2 microglobulin; cytotoxic
Cellular Products
immunoglobulin; monoclonal antibody; against human Beta-2 microglobulin; cytotoxic
Comments
Animals were immunized with MOLT-4 cells (ATCC CRL-1582, a human T cell line).
Spleen cells were fused with P3X63Ag8 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2b
Antigenic Determinant
Microglobulin, beta-2, human
Name of Depositor
P Parham
Deposited As
mouse (B cell); mouse (myeloma)
References

Brodsky FM, et al. Characterization of a monoclonal anti-B2-microglobulin antibody and its use in the genetic and biochemical analysis of major histocompatibility antigens. Eur. J. Immunol. 9: 536-545, 1979. PubMed: 91522

Parham P, et al. Arginine 45 is a major part of the antigenic determinant of human beta2-microglobulin recognized by mouse monoclonal antibody BBM.1. J. Biol. Chem. 258: 6179-6186, 1983. PubMed: 6189821

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