Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
neuroblastoma
Age
2 years
Gender
male
Applications
This cell line is a suitable transfection host.
Derivation
BE(2)-M17 is a clone of the SK-N-BE(2) neuroblastoma cell line (see ATCC CRL-2271) that was established in November of 1972 from a bone marrow biopsy taken from a 2 year old male with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy.
Clinical Data
Patient had repeated courses of chemotherapy and radiotherapy.
male
Comments
BE(2)-M17 cells have a reported saturation density of greater than 1 x 106 cells/cm2
The cells grow as multilayering, neuroblastic cells with occasionally long neuritic processes.
As cultures age, cells will aggregate and float.
Complete Growth Medium
The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium carefully. Add 1-2 mL trypsin (0.25%) - EDTA (0.53mM) solution and allow flasks to remain at room temperature (or incubate at 37°C) until cells detach. Add fresh culture medium, triturate cells and dispense into new culture flasks.
Subcultivation ratio: 1:20 to 1:50 at 1 to 2 week intervals is recommended Medium Renewal: Every 4 to 7 days. pH should be less than or equal to 7.3.
