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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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BE29G1
BE29G1
規格:
價格:詢價
編號:B164021
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
BE29G1
商品貨號
B164021
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antiboy is useful for studies of inflammation, lymphocyte differentiation and lymphocyte activation.
It can be used for purification of ICAM-1 and for staining of cell surface ICAM-1 by flow cytometry.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Clinical Data
The antiboy is useful for studies of inflammation, lymphocyte differentiation and lymphocyte activation.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse ICAM-1 (intercellular adhesion molecule); CD54
Cellular Products
immunoglobulin; monoclonal antibody; against mouse ICAM-1 (intercellular adhesion molecule); CD54
Comments
Animals were immunized with ICAM-1 transfected FT16.I1 cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody blocks the binding of lymphocyte function antigen 1 (LFA-1, CD11a, CD18) to ICAM-1.
The antiboy is useful for studies of inflammation, lymphocyte differentiation and lymphocyte activation.
It can be used for purification of ICAM-1 and for staining of cell surface ICAM-1 by flow cytometry.
Complete Growth Medium
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype
IgG2a
Name of Depositor
AA Brian
Deposited As
rat (B cell); mouse (myeloma)
References

Kuhlman P, et al. The accessory function of murine intercellular adhesion molecule-1 in T lymphocyte activation. Contributions of adhesion and co-activation. J. Immunol. 146: 1773-1782, 1991. PubMed: 1672331

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