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Beta-TC-6
Beta-TC-6
規(guī)格:
價格:詢價
編號:B164028
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
Beta-TC-6
商品貨號
B164028
Organism
Mus musculus, transgenic for SV40 large T antigen, mouse, transgenic for SV40 large T antigen
Tissue
pancreas
培養(yǎng)基
Cell Type
beta cell
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
2 [Cells contain SV40 viral DNA Sequences]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
insulinoma
Storage Conditions
liquid nitrogen vapor phase
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images
Derivation
The cell line was derived from a pancreatic tumor (insulinoma) arising in a transgenic mouse.
Genes Expressed
insulin, glucagon, and somatostatin
Cellular Products
insulin, glucagon and somatostatin
Comments
They secrete insulin in response to glucose.
The mouse carried the pseudogene construct composed of the SV40 early region controlled by the rat insulin II gene promotor.
The cells contain abundant insulin and small amounts of glucagon and somatostatin. They secrete insulin in response to glucose.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes. Discard supernatant and resuspend cells in fresh growth medium.
    Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Name of Depositor
CytoTherapeutics, Inc.
Deposited As
mouse, transgenic for SV40 large T antigen
U.S. Patent Number
References

Laurance ME, et al. Glucose responsive insulin secreting beta-cell lines and method for producing same. US Patent 5,773,255 dated Jun 30 1998

Poitout V, et al. Morphological and functional characterization of beta TC-6 cells--an insulin-secreting cell line derived from transgenic mice. Diabetes 44: 306-313, 1995. PubMed: 7533732

Poitout V, et al. Insulin-secreting cell lines: classification, characteristics and potential applications. Diabetes Metab. 22: 7-14, 1996. PubMed: 8697299

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