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C1R-B7
C1R-B7
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B164076
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
C1R-B7
商品貨號(hào)
B164076
Organism
Homo sapiens, human
Cell Type
B lymphoblast; Epstein-Barr virus (EBV) transforme
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
mixed; semi-attached
Biosafety Level
2 Cells contain herpesvirus

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
It expresses surface HLA B7 but does not secrete HLA B7.
C1R-B7 is a stable transfectant cell line established in 1988 with a normal major histocompatibility complex (MHC) HLA B7 gene.
B-LCL by gamma irradiation followed by selection for Class I monoclonal antibodies and complement.
C1R is a human B-cell lymphoblastoid line lacking surface HLA A and B antigens.
C1R was derived from Hmy.
C1R-B7 may be used as a control for C1R-sB7; in CTL experiments and for producing control supernatants.
Derivation
C1R-B7 is a stable transfectant cell line established in 1988 with a normal major histocompatibility complex (MHC) HLA B7 gene. It expresses surface HLA B7 but does not secrete HLA B7.
C1R is a human B-cell lymphoblastoid line lacking surface HLA A and B antigens. C1R was derived from Hmy.2 B-LCL by gamma irradiation followed by selection for Class I monoclonal antibodies and complement.
Antigen Expression
Expresses surface HLA B7; does not secrete HLA B7
Genes Expressed
Expresses surface HLA B7; does not secrete HLA B7
Comments
C1R-B7 is a stable transfectant cell line established in 1988 with a normal major histocompatibility complex (MHC) HLA B7 gene. It expresses surface HLA B7 but does not secrete HLA B7.
C1R is a human B-cell lymphoblastoid line lacking surface HLA A and B antigens. C1R was derived from Hmy.2 B-LCL by gamma irradiation followed by selection for Class I monoclonal antibodies and complement.
C1R-B7 may be used as a control for C1R-sB7; in CTL experiments and for producing control supernatants.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by transferring floating cells to additional flasks.
Attached cells may be subcultured by scraping.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 6,10
D13S317: 11,13
D16S539: 9,13
D5S818: 10,13
D7S820: 7,12
THO1: 8
TPOX: 8
vWA: 17
Name of Depositor
F Grumet
Year of Origin
1988
References

Storkus WJ, et al. Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes. Proc. Natl. Acad. Sci. USA 86: 2361-2364, 1989. PubMed: 2784569

Grumet FC, et al. Soluble form of an HLA-B7 class I antigen specifically suppresses humoral alloimmunization. Hum. Immunol. 40: 228-234, 1994. PubMed: 7960967

Hiraki DD, et al. Bioengineered soluble HLA-B7. Genesis, characterization, and occurrence of dimerization. Hum. Immunol. 40: 235-246, 1994. PubMed: 7960968

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