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當前位置: 首頁 > ATCC代理 > C-33A [c-33a] B164085
C-33A [c-33a]
C-33A [c-33a]
規格:
價格:詢價
編號:B164085
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
C-33A [c-33a]
商品貨號
B164085
Organism
Homo sapiens, human
Tissue
cervix
培養基
Cell Type
Epithelial, Retinoblastoma
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
carcinoma
Age
66 years adult
Gender
female
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
This a pseudodiploid human cell line with the modal chromosome number of 46, occurring in 70% of cells examined. Polyploid cells occurred at 8.6%. Seven marker chromosomes were consistently detected per pseudodiploid cell. They are: t(1q17q), t(1p21q), del(18) (q21.3), der(1)t(1;17) (p16;q21.3) and three others. Several other markers were also found but they occurred only once in 15 metaphases analyzed. Neither DMs nor HSRs were detected. Structurally normal N1 was absent. Generally there are two X chromosomes in each cell.
Clinical Data
66 years adult
Caucasian
female
Oncogene
p53 +; pRB +
Genes Expressed

Oncogenes: p53 +; pRB +

Tumorigenic
Yes
Effects
Yes, in nude mice; forms undifferentiated carcinoma
Comments
The line exhibited a hypodiploid karyotype initially, and an epithelial morphology.
Karyological instability was observed with continued passage.

The retinoblastoma protein (pRB) is present but abnormal in size. 

Expression of p53 is elevated, and there is a point mutation at codon 273 resulting in a Arg -> Cys substitution.

The cells are negative for human papillomavirus DNA and RNA.

The C-33 A cell line is one of a series of lines (see also ATCC CRL-1594 and ATCC CRL-1595) derived by N. Auersperg from cervical cancer biopsies.

Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 12
D13S317: 13
D16S539: 13,14
D5S818: 11,12
D7S820: 10
THO1: 7,8
TPOX: 9
vWA: 18,20
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1
PGM3, 1
Name of Depositor
N Auersperg
Deposited As
Homo sapiens
Passage History
The C-33 A cell line is one of a series of lines (see also ATCC CRL-1594 and ATCC CRL-1595) derived by N. Auersperg from cervical cancer biopsies. The line exhibited a hypodiploid karyotype initially, and an epithelial morphology. Karyological instability was observed with continued passage. The retinoblastoma protein (pRB) is present but abnormal in size. Expression of p53 is elevated, and there is a point mutation at codon 273 resulting in a Arg -> Cys substitution. The cells are negative for human papillomavirus DNA and RNA.
References

J. Natl. Cancer Inst. 32: 135-148, 1964.

Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217

Scheffner M, et al. The state of the p53 and retinoblastoma genes in human cervical carcinoma cell lines. Proc. Natl. Acad. Sci. USA 88: 5523-5527, 1991. PubMed: 1648218

Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

Kovelman R, et al. Enhanced transcriptional activation by E2 proteins from the oncogenic human papillomaviruses. J. Virol. 70: 7549-7560, 1996. PubMed: 8892874

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