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CC56
CC56
規格:
價格:詢價
編號:B164137
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CC56
商品貨號
B164137
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with bovine B cells in peripheral blood and lymph nodes.
Derivation
Spleen cells were fused with NS1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine B cell
Cellular Products
immunoglobulin; monoclonal antibody; against bovine B cell
Comments
Animals were immunized with bovine mesenteric lymph node cells.
Spleen cells were fused with NS1 myeloma cells.
The antibody reacts with bovine B cells in peripheral blood and lymph nodes.
It precipitates a 180000 dalton molecule from the surface of bovine B cells.
This antibody was included in the panel of the International Workshop on Bovine, Sheep and Goat Leukocyte Differentiation Antigens in 1989 and 1992.
Complete Growth Medium
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG2a
Name of Depositor
CJ Howard
Deposited As
mouse (B cell); mouse (myeloma)
References

Hein WR, et al. Epitopes of the T19 lymphocyte surface antigen are extensively conserved in ruminants. Vet. Immunol. Immunopathol. 27: 173-181, 1991. PubMed: 1708546

. . Vet. Immunol. Immunopathol. 32: 1993..

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