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CCD-1099Sk
CCD-1099Sk
規(guī)格:
價格:詢價
編號:B164170
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
CCD-1099Sk
商品貨號
B164170
Organism
Homo sapiens, human
Cell Type
fibroblast
培養(yǎng)基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Age
24 years
Gender
male
Ethnicity
Caucasian
Applications
The line was established from normal skin from the leg.
Derivation
The line was established from normal skin from the leg.
Clinical Data
male
Caucasian
24 years
Comments
The line was established from normal skin from the leg.
Cells senesce after approximately 60 population doublings.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor
H.White, ATCC
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