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CCRF S-180 II
CCRF S-180 II
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B164216
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
CCRF S-180 II
商品貨號(hào)
B164216
Organism
Mus musculus, mouse
Product Format
frozen
培養(yǎng)基
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
sarcoma
Age
adult
Strain
CFW
Applications
Tested and found negative for ectromelia virus (mousepox).
Tumorigenic
Yes
Effects
Yes, in CFW mice
Virus Resistance
poliovirus 1
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids, 95%; bovine calf serum, 5%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 2 to 5 minutes, remove trypsin and add fresh medium. Aspirate cells and dispense into new flasks.
Name of Depositor
GE Foley
Deposited As
Mus musculus
References

Foley GE, et al. Isolation and serial propagation of malignant and normal cells in semi-defined media. Origins of CCRF cell lines. Cancer Res. 20: 930-939, 1960. PubMed: 13823721

Foley EG, Drolet BP. Sustained propagation of sarcoma of 180 in tissue culture. Proc. Soc. Exp. Biol. Med. 92: 347-352, 1956. PubMed: 13350343

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