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CDR1
CDR1
規(guī)格:
價格:詢價
編號:B164221
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
CDR1
商品貨號
B164221
Organism
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
It does not react with rat or human thymus, but does cross-reacts with epithelial elements in mouse kidney, large and small intestine, brain and spleen vasculature, and human skin.
Derivation
Spleen cells were fused with Sp2/0 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against mouse cortical thymic epithelium and a 160000 dalton Abelson leukemia antigen
Cellular Products
immunoglobulin; monoclonal antibody; against mouse cortical thymic epithelium and a 160000 dalton Abelson leukemia antigen
Comments
Animals were immunized with mouse thymic stromal tissue.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody does not react with the thymic medulla, and the reactivity appears to be identical to that reported for the 6C3 hybridoma (Proc. Natl. Acad. Sci. USA 81:4434-4438, 1984).
It does not react with rat or human thymus, but does cross-reacts with epithelial elements in mouse kidney, large and small intestine, brain and spleen vasculature, and human skin.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Isotype
IgG2a
Antigenic Determinant
Abelson murine leukemia virus, 16-kDa antigen
Name of Depositor
RV Rouse
Deposited As
rat (B cell); mouse (myeloma)
References

Pillemer E, et al. Transformation-associated proteins in murine B-cell lymphomas that are distinct from Abelson virus gene products. Proc. Natl. Acad. Sci. USA 81: 4434-4438, 1984. PubMed: 6611551

Rouse RV, et al. Monoclonal antibodies reactive with subsets of mouse and human thymic epithelial cells. J. Histochem. Cytochem. 36: 1511-1517, 1988. PubMed: 2461413

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