Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
xeroderma pigmentosum, presumed heterozygote
Age
54 years
Gender
female
Clinical Data
The tissue donor was a presumed heterozygote for xeroderma pigmentosum, and was the mother of Ge Ar (ATCC CRL-1161) and Em Ar (ATCC CRL-1168).
female
Comments
The tissue donor was a presumed heterozygote for xeroderma pigmentosum, and was the mother of Ge Ar (ATCC CRL-1161) and Em Ar (ATCC CRL-1168).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 7 to 9 days.
