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CEM-CM3
CEM-CM3
規(guī)格:
價格:詢價
編號:B164228
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
CEM-CM3
商品貨號
B164228
Organism
Homo sapiens, human
Tissue
peripheral blood
培養(yǎng)基
Cell Type
T lymphoblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
acute lymphoblastic leukemia
Age
4 years
Gender
female
Ethnicity
Caucasian
Applications
This line was derived from CCRF-CEM (see ATCC CCL-119) by selecting for resistance to 8-azaguanine.
The line has been used as a fusion partner in the production of human-human T cell hybridomas.
Storage Conditions
liquid nitogen vapor phase
Derivation
This line was derived from CCRF-CEM (see ATCC CCL-119) by selecting for resistance to 8-azaguanine.
Clinical Data
female
Caucasian
4 years
Comments
This line was derived from CCRF-CEM (see ATCC CCL-119) by selecting for resistance to 8-azaguanine.
The line has been used as a fusion partner in the production of human-human T cell hybridomas.
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.015 mg/ml 8-azaguanine, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitogen vapor phase
Culture Conditions
Temperature: 37.0°C
STR Profile
Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 10,12
D5S818: 12,13
D7S820: 10,13
THO1: 6,7
TPOX: 8
vWA: 18,19
Name of Depositor
MK Cathcart
Deposited As
Homo sapiens
References

Cathcart MK, Murakami MNonspecific suppression of antibody synthesis by products of human T lymphocyte hybridomas. Use of a newly selected parent fusion lineIn: Cathcart MK, Murakami MT cell hybridomasBoca Raton, FLCRC Presspp. 227-236, 1985

. Human T lymphocyte hybridomas: production of nonspecific suppressor factors of antibody synthesis. Kyoto, Japan: 5th International Congress of Immunology; 1983.

. . Clin. Res. 31: 734A, 1983.

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