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ChaGo-K-1
ChaGo-K-1
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B164244
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
ChaGo-K-1
商品貨號(hào)
B164244
Organism
Homo sapiens, human
Tissue
lung, bronchus
培養(yǎng)基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
bronchogenic carcinoma
Age
45 years
Gender
male
Storage Conditions
liquid nitrogen vapor phase
Karyotype
This is a hyperdiploid human cell line. The modal chromosome number is 52, occurring at 40% with polyploidy at 3.8%., Over 2/3 of the chromosome complement consist of those with structural rearrangements (or markers) including 1q, t(1p,16q), del(3)(p13), t(5;14)(q35;q11), t(9q;?)1, t(9q;?)2, 12p+, 20p+, t(Xq,7p)., Twenty-one other markers are common to most cells. The normal chromosomes 1,3,5,9,14,15 and X are absent. All three E group chromosomes are paired., Y chromosome not detected by Q staining.
Genes Expressed
hCG; human chorionic gonadotropin (alpha subunit only); estradiol; progesterone; mucin (apomucin, MUC-1, MUC-2)
Cellular Products
hCG; human chorionic gonadotropin (alpha subunit only); estradiol; progesterone; mucin (apomucin, MUC-1, MUC-2)
Comments
The cells produce high levels of MUC-1 mucin mRNA, low levels of MUC-2 mRNA but do not express the MUC-3 gene.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: 1:4 to 1:6
Medium Renewal: Every 2 to 3 days

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.

Cryopreservation
Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions

Temperature: 37°C
Atmosphere: 5% CO2 

STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 8,12
THO1: 9.3
TPOX: 8
vWA: 17,18
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
LDH, 5
Me-2, 0
PGM1, 1
PGM3, 1
Name of Depositor
S Lippman, A Rabson
Deposited As
Homo sapiens
References

Lieblich JM, et al. Ectopic and eutopic secretion of chorionic gonadotropin and its subunits in vitro: Comparison of clonal strains from carcinomas of lung and placenta. J. Natl. Cancer Inst. 56: 911-917, 1976. PubMed: 62839

Rabson AS, et al. Production of human chorionic gonadotropin in vitro by a cell line derived from a carcinoma of the lung. J. Natl. Cancer Inst. 50: 669-674, 1973. PubMed: 4708151

Dahiya R, et al. Mucin synthesis and secretion in various human epithelial cancer cell lines that express the MUC-1 mucin gene. Cancer Res. 53: 1437-1443, 1993. PubMed: 8443822

Tashjian AH Jr., et al. Subunits of human chorionic gonadotropin: unbalanced synthesis and secretion by clonal cell strains derived from a bronchogenic carcinoma. Proc. Natl. Acad. Sci. USA 70: 1419-1422, 1973. PubMed: 4514312

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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