Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Expresses human tissue plasminogen activator (t-PA) in eukaryotic DHFR deficient cell lines grown in selective media (glycine, hypoxanthine, thymidine deficient). (Goeddel DV, et al. Human tissue plasminogen activator. US Patent 4,766,075 dated Aug 23 1988)
Contains a EcoRI/BamHI fragment of about 2.2 kb containing t-PA and a 1.7 kb SacII fragment containing wild-type dihydrofolate reductase under SV40 control. (Goeddel DV, et al. Human tissue plasminogen activator. US Patent 4,766,075 dated Aug 23 1988)
Subcultivation Ratio: 1:5 to 1:10
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Goeddel DV, et al. Human tissue plasminogen activator. US Patent 4,766,075 dated Aug 23 1988
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
