黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > CMH1a B164277
CMH1a
CMH1a
規格:
價格:詢價
編號:B164277
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
CMH1a
商品貨號
B164277
Organism
Mus musculus, mouse
Tissue
mammary gland
培養基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Papilloma, hepatitis
Strain
RIII
Applications
This cell line was derived from the C127I (see ATCC CRL-1616) mouse cell line by transformation with a bovine papilloma virus vector (pRB-1) containing the DNA sequence encoding HBsAg.
HBsAg production is under the control of the metallothionine promoter.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
This cell line was derived from the C127I (see ATCC CRL-1616) mouse cell line by transformation with a bovine papilloma virus vector (pRB-1) containing the DNA sequence encoding HBsAg.
HeLa Markers
N
Genes Expressed
surface antigen [sAg]
Comments
This cell line was derived from the C127I (see ATCC CRL-1616) mouse cell line by transformation with a bovine papilloma virus vector (pRB-1) containing the DNA sequence encoding HBsAg.
HBsAg production is under the control of the metallothionine promoter.
The construct contains the entire BPV genome and the hepatitis B sAg structural gene inserted within the MT gene between the promoter and the rest of the gene.
A BamHI/SalI fragment of pRF300 (containing hepatitis B sAg and MT-1 promoter) was ligated to a BamHI/SalI fragment of pB2-2 (containing the entire BPV genome and pBR sequences) to create pRB-1.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:15 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature (or 37C) until cells detach (about 10 minutes). Add fresh medium, aspirate and dispense, into new flasks.
Cryopreservation
fetal bovine serum, 90%; glycerol, 10%
Culture Conditions
Temperature: 37.0°C
Name of Depositor
Integrated Genetics, Inc.
Deposited As
Mus musculus
U.S. Patent Number
References

Fitts RA, et al. Vector encoding hepatitis B surface antigen. US Patent 4,496,898 dated Sep 29 1987

  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479
  • 曹經理
    手機號：18662422060
    QQ：3671869992