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CPA 47
CPA 47
規(guī)格:
價格:詢價
編號:B164291
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
CPA 47
商品貨號
B164291
Organism
Bos taurus, cow
Tissue
pulmonary artery, vascular endothelium
培養(yǎng)基
Cell Type
endothelial
Product Format
frozen
Morphology
cobblestone
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
normal
Age
calf
Storage Conditions
liquid nitrogen vapor phase
Derivation
The CPA 47 cell line was established from cells scraped from the luminal surface of the pulmonary artery.
Genes Expressed
Tested and found negative for ectromelia virus (mousepox).
factor VIII; angiotensin converting enzyme,Recent tests for bovine viral diarrhea (BVD) virus indicate that CPA 47 cells are positive for both BVD antigen and infectious virions when the culture medium contains serum of bovine origin.
Cellular Products
factor VIII; angiotensin converting enzyme
Comments
The cells have never been exposed to proteolytic enzymes, and therefore possess an intact glycocalyx.

CPA 47 cells show cobblestone morphology, and are positive for factor VIII and angiotensin converting enzyme (ACE).

The cells have a life expectancy of about 5 years (120 passages), and can be grown on microcarriers.

Recent tests for bovine viral diarrhea (BVD) virus indicate that CPA 47 cells are positive for both BVD antigen and infectious virions when the culture medium contains serum of bovine origin.

Current lots have been propagated using horse serum and all tests for BVD virus were negative.
Complete Growth Medium
Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 90%; horse serum, 10%
Subculturing
Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the substrate, aspirate and dispense into new flasks new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended.
Medium Renewal: Twice per week
Cryopreservation
culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37°C
Name of Depositor
US Ryan
Deposited As
Bos taurus
Passage History
The cells have a life expectancy of about 5 years (120 passages) and can be grown on microcarriers.
References

Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

Ryan US, et al. Isolation and culture of pulmonary artery endothelial cells. Tissue Cell 10: 535-554, 1978. PubMed: 83028

Ryan US, et al. Methods for microcarrier culture of bovine pulmonary artery endothelial cells avoiding the use of enzymes. Tissue Cell 12: 619-635, 1980. PubMed: 7010675

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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