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D1B
D1B
規格:
價格:詢價
編號:B164315
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
D1B
商品貨號
B164315
Organism
Mus musculus, mouse
Tissue
spleen
培養基
Cell Type
erythroblast
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
leukemia
Strain
DBA/2
Applications
This line was derived from a leukemia induced by B-tropic Friend murine leukemia virus.
The cells are negative for H-2d (H-2d-), surface immunoglobulin (sIg-), Ia8 (Ia8-) and Thy-1.2 (Thy-1.2-).
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions
liquid nitrogen vapor phase
Derivation
This line was derived from a leukemia induced by B-tropic Friend murine leukemia virus.
Genes Expressed
globin mRNA; hemoglobin
Cellular Products
globin mRNA; hemoglobin
Tumorigenic
Yes
Effects
Yes, in DBA/2 mice
Comments
This line was derived from a leukemia induced by B-tropic Friend murine leukemia virus.
The cells are negative for H-2d (H-2d-), surface immunoglobulin (sIg-), Ia8 (Ia8-) and Thy-1.2 (Thy-1.2-).
Erythroid differentiation and hemoglobin synthesis are inducible by DMSO.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.01 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10 exp6 cells/ml. The cells may also be grown as ascites in syngeneic mice.Inject 2 X 10(5) cell per mouse and harvest as soon as abdominal enlargement is observed (about 10 days).
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Name of Depositor
B Chesebro
Deposited As
Mus musculus
References

Chesebro B, et al. Lack of erythroid characteristics in Ia-positive leukemia cell lines induced by Friend murine leukemia virus: brief communication. J. Natl. Cancer Inst. 60: 239-242, 1978. PubMed: 564410

Chesebro B, et al. Characterization of Ia8 antigen, thy-1.2 antigen, complement receptors, and virus production in a group of murine virus-induced leukemia cell lines. J. Immunol. 117: 1267-1274, 1976. PubMed: 185295

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