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DSDh
DSDh
規格:
價格:
編號:B164372
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
DSDh
商品貨號
B164372
Organism
Canis familiaris, dog
Tissue
bone
培養基
Product Format
frozen
Morphology
fibroblast
Culture Properties
adherent
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
osteosarcoma
Age
11 years
Gender
female
Strain
poodle
Applications
DSDh cells were isolated after selection with methotrexate and screening for helper activity.
The line is used as a helper cell for propagating replication incompetent vectors derived from spleen necrosis virus (SNV).
After serial passaging for 2 months, the cells appears to decrease in helper activity (as evidence by drops in viral titers produced), thus it is best to prepare generous frozen stocks of the cells upon receipt.
Derivation
DSDh cells were isolated after selection with methotrexate and screening for helper activity.
The line is used as a helper cell for propagating replication incompetent vectors derived from spleen necrosis virus (SNV).
After serial passaging for 2 months, the cells appears to decrease in helper activity (as evidence by drops in viral titers produced), thus it is best to prepare generous frozen stocks of the cells upon receipt.
Clinical Data
female
Comments
DSDh is a retrovirus packaging cell line derive from the D-17 canine osteogenic sarcoma cell line (ATCC CCL-183) by Howard Temin.
D-17 cells were transfected with plasmids pBR1 (gag - pol genes from spleen necrosis virus), pPR102 (env gene from spleen necrosis virus) and pFR400 (dihydrofolate reductase gene).
DSDh cells were isolated after selection with methotrexate and screening for helper activity.
The line is used as a helper cell for propagating replication incompetent vectors derived from spleen necrosis virus (SNV).
After serial passaging for 2 months, the cells appears to decrease in helper activity (as evidence by drops in viral titers produced), thus it is best to prepare generous frozen stocks of the cells upon receipt.
Complete Growth Medium
Minimum essential medium (Eagle) with Earle's BSS containing 500 nM methotrexate, 92%; fetal bovine serum, 8%
Subculturing
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Subculture at or prior to becoming confluent.
Inoculate new flasks with 1 to 2 X 10 exp5 cells per sq cm.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor
H Temin, DW Burns
Deposited As
Canis familiaris
References

Hu WS, Temin HM. Genetic consequences of packaging two RNA genomes in one retroviral particle: pseudodiploidy and high rate of genetic recombination. Proc. Natl. Acad. Sci. USA 87: 1556-1560, 1990. PubMed: 2304918

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