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EL 1
EL 1
規(guī)格:
價(jià)格:詢(xún)價(jià)
編號(hào):B164402
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng)
EL 1
商品貨號(hào)
B164402
Organism
Homo sapiens, human
Tissue
spleen
培養(yǎng)基
Product Format
frozen
Morphology
monocyte/macrophage
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
The EL 1 cell line was established from cryopreserved human spleen cells.
Comments
The cells tend to grow as single cells but may form small nonadherent clusters.
Complete Growth Medium
Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; calf serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days (or as necessary to maintain the cell concentration between 2 X 10 exp5 and 1 X 10 exp6 cells/ml)
Cultures can be maintained by addition or replacement of fresh medium. Maintain the cell concentration between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
STR Profile

This cell line was originally deposited in support of a patent (US Patent 5,447,861).

STR profiling at ATCC indicates similarity to other cell lines including:
U-937 (ATCC CRL-1593.2) and
SC (ATCC CRL-9855)
Name of Depositor
E.I. du Pont de Nemours & Co.
Deposited As
Homo sapiens
U.S. Patent Number
References

Collins GW, Largen MT. Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995

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