Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
A subline (EL4.IL-2, ATCC TIB-181) that produces high levels of interleukin-2 (IL-2, interleukin 2) is available.
A subline (EL4.IL-2, ATCC TIB-40) that is resistant to 0.1 mM 5-bromo-2'-deoxyuridine (BUdR) is available.
A subline (EL4.BU.1.OUAr.1.1, ATCC TIB-41) that is resistant to 0.1 mM 5-bromo-2'-deoxyuridine and 1 mM ouabain is available.
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL. Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product.
Medium Renewal: Every 2 to 3 days
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