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EOMA-GFP
EOMA-GFP
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B164414
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
EOMA-GFP
商品貨號(hào)
B164414
Organism
Mus musculus, mouse
Cell Type
endothelial
培養(yǎng)基
Product Format
frozen
Morphology
endothelial
Culture Properties
adherent
Biosafety Level
2 Cells contain SV40 and HSV viral sequences

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
hemangioendothelioma
Age
adult
Applications
The EOMA-GFP cell line was derived i 1998 from the EOMA cell line (ATCC CRL-2586) by transfection with a plasmid reporter vector, pEGFP-N1, encoding enhanced green fluorescent protein (GFP).
The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an adult mouse.
Storage Conditions
liquid nitrogen vapor phase
Derivation
The EOMA-GFP cell line was derived i 1998 from the EOMA cell line (ATCC CRL-2586) by transfection with a plasmid reporter vector, pEGFP-N1, encoding enhanced green fluorescent protein (GFP). The vector was obtained from Clontech, Palo Alto, CA (#6085-1).
The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an adult mouse.
Comments
The EOMA-GFP cell line was derived i 1998 from the EOMA cell line (ATCC CRL-2586) by transfection with a plasmid reporter vector, pEGFP-N1, encoding enhanced green fluorescent protein (GFP). The vector was obtained from Clontech, Palo Alto, CA (#6085-1).
The vector contains cytomegalovirus (CMV), SV40 and Herpes simplex virus (HSV) viral DNA sequences and the neomycin resistance gene.
The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an adult mouse.
For additional details see EOMA (ATCC CRL-2586).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • 0.2 mg/ml G -418
  • fetal bovine serum to a final concentration of 10%
    • Subculturing
    Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
    Subcultivation Ratio: A subcultivation ratio of 1:6 is recommended
    Medium Renewal: Every 2 to 3 days
    Cryopreservation
    Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor phase
    Culture Conditions
    Temperature: 37.0°C
    Name of Depositor
    R Auerbach
    Deposited As
    mouse
    Year of Origin
    1998
    References

    Obeso J, et al. A hemangioendothelioma-derived cell line: its use as a model for the study of endothelial cell biology. Lab. Invest. 63: 259-269, 1990. PubMed: 2166185

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