Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006).To make the complete growth medium, add the following components to the base medium:
The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium:
Subculturing
The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium:
Cryopreservation
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended every 10 to 15 days
Medium Renewal: Every 3 to 4 days.
Culture Conditions
Freeze Medium: Complete growth medium supplemented with an extra 50% FBS and 10% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase
STR Profile
Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Name of Depositor
Amelogenin: X,Y CSF1PO: 11,12 D13S317: 12,13 D16S539: 9,11 D5S818: 12,13 D7S820: 8,12 THO1: 6,9.3 TPOX: 8,11 vWA: 16
Deposited As
DP Chopra
References
Homo sapiens
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