Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Minimum essential medium (Eagle) with Hank’s balanced salts (Life Technologies Cat# 11575-032), 90%; fetal bovine serum, 10%
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Gravell M, Malsberger RG. A permanent cell line from the fathead minnow (Pimephales promelas). Ann. N.Y. Acad. Sci. 126: 555-565, 1965. PubMed: 5220177
Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am. J. Hyg. 78: 173-180, 1963. PubMed: 14063721
