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FL74-UCD-1
FL74-UCD-1
規格:
價格:詢價
編號:B164465
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
FL74-UCD-1
商品貨號
B164465
Organism
Felis catus, cat
Cell Type
lymphoblast
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
leukemia
Applications
The cells are capable of producing large amounts of feline leukemia that has attenuated virulence for cats, but which is capable of being used for vaccine preparation.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
The line was established from the lymphomatous kidney of a cat that had been inoculated with virus obtained by serial cell free passage of virus from a cat with spontaneous leukemia.
Genes Expressed
Feline leukemia virus (FeLV), KT-FeLV-UCD-1 strain
Cellular Products
Feline leukemia virus (FeLV), KT-FeLV-UCD-1 strain
Comments
These cells are persistently infected with the KT-FeLV-UCD-1 feline leukemia virus.
The line was established from the lymphomatous kidney of a cat that had been inoculated with virus obtained by serial cell free passage of virus from a cat with spontaneous leukemia.
The cells are capable of producing large amounts of feline leukemia that has attenuated virulence for cats, but which is capable of being used for vaccine preparation.
Complete Growth Medium
Leibovitz's L-15 medium with 2mM L-glutamine, 85%; fetal bovine serum, 15%

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation).


Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor
NC Pedersen
Deposited As
Felis catus
U.S. Patent Number
Passage History
The line was established from the lymphomatous kidney of a cat that had been inoculated with virus obtained by serial cell free passage of virus from a cat with spontaneous leukemia.
References

Pedersen NC, Theilen GH. Vaccine for preventing persistent feline leukemia viremia in cats. US Patent 4,264,587 dated Apr 28 1981

Theilen GH, et al. Replication of cat leukemia virus in cell suspension cultures. Nature 222: 589-590, 1969. PubMed: 5818677

Pedersen NC, et al. Safety and efficacy studies of live- and killed-feline leukemia virus vaccines. Am. J. Vet. Res. 40: 1120-1126, 1979. PubMed: 230767

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