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They differentiate to a neuronal phenotype at the nonpermissive temperature (39°C) when induced by basic fibroblast growth factor (bFGF) in N2 medium (DMEM-high glucose medium with supplements).
At 39°C, expression of the human IGF-IR in H19-7 cells induces an insulin-like growth factor (IGF) I dependent differentiation. The cells extend neurites and show increased expression of NF68.
H19-7/IGF-IR cells express the IGF-IR protein. IGF-IR is known to send two seemingly contradictory signals inducing either cell proliferation or cell differentiation, depending on cell type and/or conditions.
Morrione A, et al. Insulin-like growth factor I receptor signaling in differentiation of neuronal H19-7 cells. Cancer Res. 60: 2263-2272, 2000. PubMed: 10786694
Eves EM, et al. Immortal rat hippocampal cell lines exhibit neuronal and glial lineages and neurotrophin gene expression. Proc. Natl. Acad. Sci. USA 89: 4373-4377, 1992. PubMed: 1316607
