黄色小说视频-日本少妇高潮抽搐-国内自拍av-天堂中文资源在线-蜜桃视频在线入口www-91久久综合-亚洲视频在线一区-日日夜夜拍-国产精品资源在线观看-欧美激情国产精品免费-人妻少妇久久中文字幕-成人免费无码大片a毛片-香蕉污视频在线观看-日本电影成人-国内精品久久99人妻无码

歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > H1HeLa B164526
H1HeLa
H1HeLa
規格:
價格:
編號:B164526
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
H1HeLa
商品貨號
B164526
Organism
Homo sapiens, human
Tissue
cervix
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
2  [Cells contain human papilloma virus (HPV-18)]

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
adenocarcinoma
Age
31 years
Gender
female
Ethnicity
Black
Applications
The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al.
This subline is susceptible to rhinoviruses and is useful for passaging and titrating rhinoviruses.
Derivation
The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al. (see ATCC CCL-2).
Clinical Data
31 years
Black
female
HeLa Markers
Y
Genes Expressed
keratin
Cellular Products
keratin
Comments
The H-Hela cell line was established by V. Hamparian from the HeLa cell line isolated by Gey, et al. (see ATCC CCL-2).
This subline is susceptible to rhinoviruses and is useful for passaging and titrating rhinoviruses.
The line is usually carried as monolayer cultures in Leibovitz's L-15 medium, but can be readily adapted to other media and to suspension culture (in media without Ca++).
The H-HeLa line was cured of a mycoplasma contamination by W.M. Lee in Rueckert's laboratory and the cured line was given the designation H1-HeLa.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation)


Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Remove the medium and add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Allow the culture to sit for 5 minutes at room temperature. Add fresh culture medium, aspirate and dispense into new flasks.
Culture Conditions

Atmosphere: air, 100%

Temperature: 37°C

STR Profile
Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes
G6PD, A
Name of Depositor
RR Rueckert
Deposited As
Homo sapiens
References

Lee WM Ph.D. thesis, Univ. Wisconsin, 1991

Medappa KC, et al. On the structure of rhinovirus 1A. Virology 44: 259-270, 1971. PubMed: 4327717

  • 王經理
    手機號：17280875617
    QQ：1438578920
  • 胡經理
    手機號：13345964880
    QQ：2438244627
  • 周經理
    手機號：17757487661
    QQ：1296385441
  • 于經理
    手機號：18067160830
    QQ：2088210172
  • 沈經理
    手機號：19548299266
    QQ：2662369050
  • 李經理
    手機號：13626845108
    QQ：972239479