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HP6047
HP6047
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B164639
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
HP6047
商品貨號(hào)
B164639
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension, but with many adherent cells
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with hinge region epitopes that are different from those reactive with HP6050 (ATCC CRL-1768).
Tested and found negative for ectromelia virus (mousepox).
Derivation
Animals were immunized with purified human myeloma protein.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human IgG3 hinge region
Cellular Products
immunoglobulin; monoclonal antibody; against human IgG3 hinge region
Tumorigenic
Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with purified human myeloma protein.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with hinge region epitopes that are different from those reactive with HP6050 (ATCC CRL-1768).
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.01 mM nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium.
Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1
Name of Depositor
CB Reimer
Deposited As
mouse (B cell); mouse (myeloma)
References

Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201

Jefferis R, et al. Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol. Lett. 10: 223-252, 1985. PubMed: 3899923

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