Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
ATCC HTB-140 (Hs 294T) and ATCC CRL-7898 (A101D) were isolated from the same donor tissue.
The proliferative rate of the cell line is enhanced by addition of retinoic acid.
Responds to mitogenic stimulation by phytohemagglutinin and forms colonies in soft agar medium or on contact inhibited monolayers. The melanoma cells have also been shown to be responsive to the anti proliferative activity of interferon.
Results from a competitive binding study indicated that Hs 294T cells possess at least two types of interferon (IFN) receptors, one which binds IFN alpha and IFN beta and another which binds IFN gamma.
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37°C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
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Creasey AA, et al. Role of G0-G1 arrest in the inhibition of tumor cell growth by interferon. Proc. Natl. Acad. Sci. USA 77: 1471-1475, 1980. PubMed: 6154934
Helson L. Phytohemagglutinin effects on cultured human neural crest tumors. In Vitro 15: 565-568, 1979. PubMed: 511204
Fabricant RN, et al. Nerve growth factor receptors on human melanoma cells in culture. Proc. Natl. Acad. Sci. USA 74: 565-569, 1977. PubMed: 265522
Creasey AA, et al. Biological properties of human melanoma cells in culture. In Vitro 15: 342-350, 1979. PubMed: 478563
Lotan R. Different susceptibilities of human melanoma and breast carcinoma cell lines to retinoic acid-induced growth inhibition. Cancer Res. 39: 1014-1019, 1979. PubMed: 427741
Nucleotide (GenBank) : U02329 Human clone ndf294 neu differentiation factor mRNA, partial cds.
