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Hs 746T
Hs 746T
規格:
價格:詢價
編號:B164733
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
Hs 746T
商品貨號
B164733
Organism
Homo sapiens, human
Tissue
stomach; derived from metastatic site: left leg
培養基
Cell Type
Epithelial,fibroblast
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
gastric carcinoma
Age
74 years adult
Gender
male
Ethnicity
Caucasian
Applications
Hs 746T exhibited gross alteration in size, shape and staining of cells with bizarre nuclear and nucleolar shapes.
Colonies formed in Methocel and on confluent monolayers of normal human epithelial cells and fibroblasts.
Storage Conditions
liquid nitrogen vapor phase
Karyotype
This is a hypertriploid human cell line having the modal chromosome number of 77 occurring in 46% of cells. Cells with higher ploidies were found at 16.6%. The karyotype of this cell line is very complex. Between 45% and 55% of chromosomes in each cell had structural aberrations, including: der(4)t(1;4) (p13.1;q35); t(15q22q); del(2) (p13); der(7)t(7;?) (p22;?) [7p+]; t(7q22q?) and others. The t(15q22q), del(2), and t(7q22q?) (and several others) had two copies in most cells. There were two types of der(7): one was the 7p+ described above; the other, 7p++, was distinctly longer than N1. There were more than ten markers that were present in some cells only. One DM-like chromosome was found in most cells. Normal N7 and N21 were absent. One normal X and one or two normal Y chromosomes were detected.
Clinical Data
74 years adult
Caucasian
male
Tumorigenic
Yes
Effects
Yes, in immunosuppressed mice
Comments
In vitro transformation properties were assayed at various early passages. Hs 746T exhibited gross alteration in size, shape and staining of cells with bizarre nuclear and nucleolar shapes. Colonies formed in Methocel and on confluent monolayers of normal human epithelial cells and fibroblasts.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10
D16S539: 11
D5S818: 11,13
D7S820: 8
THO1: 9
TPOX: 8,10
vWA: 15,17
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 1
PGM1, 1-2
PGM3, 1
Name of Depositor
RB Owens
Deposited As
Homo sapiens
Passage History
In vitro transformation properties were assayed at various early passages. Hs 746T exhibited gross alteration in size, shape and staining of cells with bizarre nuclear and nucleolar shapes. Colonies formed in Methocel and on confluent monolayers of normal human epithelial cells and fibroblasts.
Year of Origin
1973
References

Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258

The cells form carcinomas.

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