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huFasM38
huFasM38
規(guī)格:
貨期:
編號:B164805
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱
huFasM38
商品貨號
B164805
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with the extracellular domain of human Fas and blocks Fas dependent killing of Jurkat cells (a human T cell line).
It inhibits the binding of other Fas specific antibodies, and inhibits Fas-L mediated lysis of T cell targets.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human Fas antigen
Cellular Products
immunoglobulin; monoclonal antibody; against human Fas antigen
Comments
Animals were immunized with a fusion protein containing the extracellular protion of human Fas and human IgG1 Fc region.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with the extracellular domain of human Fas and blocks Fas dependent killing of Jurkat cells (a human T cell line).
It inhibits the binding of other Fas specific antibodies, and inhibits Fas-L mediated lysis of T cell targets.
Complete Growth Medium
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 100 units/mL penicillin, and 0.1 mg/ml streptomycin, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Culture Conditions
Temperature: 37.0°C
Isotype
IgG1
Name of Depositor
Immunex Corporation
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Lynch DH, Alderson MR. Human anti-FAS IGG1 monoclonal antibodies. US Patent 5,620,889 dated Apr 15 1997

Lynch DB, Alderson MR. Fas antagonists and uses thereof. US Patent 5,830,469 dated Nov 3 1998

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