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I1-Hybridoma
I1-Hybridoma
規格:
價格:詢價
編號:B164820
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
I1-Hybridoma
商品貨號
B164820
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Tissue
spleen
培養基
Cell Type
hybridoma: B lymphocyte
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Vesicular Stomatitis
Age
adult
Applications
The antibody reacts with the major surface glycoprotein (G-protein) of vesicular stomatitis virus, Indiana Serotype (VSV-Ind.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
Tumorigenic
Yes
Effects
Yes, intraperitoneal injection into BALB/c or (BALB/c X BALB.B)F1 mice
Comments
Animals were immunized with vesicular stomatitis virus, Indiana Serotype (VSV-Ind.).
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
The antibody reacts with the major surface glycoprotein (G-protein) of vesicular stomatitis virus, Indiana Serotype (VSV-Ind.).
Complete Growth Medium
Minimum essential medium (Eagle) with 2.0 mM L-glutamine and Hanks' BSS adjusted to contain 0.35 g/L sodium bicarbonate 80%; fetal bovine serum, 20%.
Subculturing
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Note: This medium is formulated for use in a free gas exchange with atmospheric air. A 5% CO2 in air atmosphere is detrimental to cells when using this medium for culturing.
Cryopreservation
Freeze medium: Complete growth medium supplemented with an additional 45% fetal bovine serum and 10% DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 100%
Isotype
mouse IgG2a kappa
Name of Depositor
IS Novella, DS Lyles
Deposited As
Mus musculus (B cell); Mus musculus (myeloma)
References

Lefrancios L, Lyles DS. The interaction of antibody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies. Virology 121: 157-167, 1982. PubMed: 6180550

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