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IgG-1D2
IgG-1D2
規格:
價格:詢價
編號:B164840
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
IgG-1D2
商品貨號
B164840
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
Animals were immunized against the GST-human SREBP-2 fusion protein (amino acids 48 to 403).
The antibody recognizes both the precursor and mature forms of SREBP-2 in cultured human cells, and can be used in immunoblotting and ELISA assays to study SREBP-2 in cultured human cells grown under different conditions.
Storage Conditions
liquid nitrogen vapor phase
Derivation
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human sterol regulatory element binding protein 2 (SREBP 2, SREBP-2)
Cellular Products
immunoglobulin; monoclonal antibody; against human sterol regulatory element binding protein 2 (SREBP 2, SREBP-2)
Comments
Animals were immunized against the GST-human SREBP-2 fusion protein (amino acids 48 to 403).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody recognizes both the precursor and mature forms of SREBP-2 in cultured human cells, and can be used in immunoblotting and ELISA assays to study SREBP-2 in cultured human cells grown under different conditions.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL.
Medium Renewal:  Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype
IgG1; kappa light chain
Name of Depositor
JL Goldstein, YK Ho
Deposited As
mouse (B cell); mouse (myeloma)
References

Janowski BA, et al. The hypocholesterolemic agent LY295427 reverses suppression of sterol regulatory element-binding protein processing mediated by oxysterols. J. Biol. Chem. 48: 45408-45416, 2001. PubMed: 11577112

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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