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IgH-2
IgH-2
規(guī)格:
價(jià)格:詢價(jià)
編號:B164848
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
IgH-2
商品貨號
B164848
Organism
iguana
Tissue
heart
培養(yǎng)基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
normal
Age
immature
Gender
male
Storage Conditions
liquid nitrogen vapor phase
Karyotype
modal number = 35; range = 30 to 64.
The karyotype is stable. There is one large marker chromosome with a terminal centromere appearing in all cells in addition to 22 minute chromosomes, 10 large chromosomes with a median centromere and 2 large chromosomes with a submedian centromere.
Virus Susceptibility
iguana
Herpes simplex virus
Pseudorabies virus
Vaccinia virus
Human poliovirus 1
Vesicular stomatitis virus
Complete Growth Medium
Eagles's Minimum Essential Medium with Hanks BSS, 2 mM L-glutamine, and 0.1 mM non-essential amino acids supplemented with 10% fetal bovine serum
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 36°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension into new culture vessels.
  6. Incubate cultures at 36°C in a closed flask without CO2 in air atmosphere.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Cryopreservation
Complete growth medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 36°C
Atmosphere: Air, 100%; no CO2
Name of Depositor
HF Clark
Deposited As
iguana
Year of Origin
August, 1966
References

Cohen MM, et al. The somatic chromosomes of 3 lizard species: Gekko gecko, Iguana iguana, and Crotaphytus collaris. Experientia 23: 769-771, 1967. PubMed: 6062904

Clark HF, et al. Characterization of reptilian cell lines established at incubation temperatures of 23 to 36 degrees. Proc. Soc. Exp. Biol. Med. 133: 1039-1047, 1970. PubMed: 4314147

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Cross References

Nucleotide (GenBank) : L27530 Iguana small cytoplasmic RNA (Y3).

Nucleotide (GenBank) : L27537 Iguana small cytoplasmic RNA (Y4).

Nucleotide (GenBank) : L27532 Iguana small cytoplasmic RNA (Y3) gene.

Nucleotide (GenBank) : L27534 Iguana small cytoplasmic RNA (Y4) gene.

Nucleotide (GenBank) : L27536 Iguana small cytoplasmic RNA (Y4) gene.

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