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ILB1-H67
ILB1-H67
規格:
價格:詢價
編號:B164856
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
ILB1-H67
商品貨號
B164856
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts specifically with IL-1 beta and does not react with IL-1 alpha or with acidic or basic fibroblast growth factor.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with purified recombinant human IL-1 beta (170500 dalton form).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human interleukin 1 beta (interleukin-1 beta, IL-1 beta)
Cellular Products
immunoglobulin; monoclonal antibody; against human interleukin 1 beta (interleukin-1 beta, IL-1 beta)
Tumorigenic
Yes
Effects
Yes, forms ascites in BALB/c mice
Comments
Animals were immunized with purified recombinant human IL-1 beta (170500 dalton form).
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts specifically with IL-1 beta and does not react with IL-1 alpha or with acidic or basic fibroblast growth factor.
It recognizes an epitope distinct from that bound by ILB1-H6 and ILB1-H34 (ATCC HB-10219 and ATCC HB-10221).
The affinity constant of ILB1-H67 was measured to be 4.8 X 10 exp10 liter/mol.
Complete Growth Medium
CULTURE MEDIUM: Dulbecco's modified Eagle's medium with 4 mM L-glutamine
adjusted to contain 1.5g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM sodium
pyruvate, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
culture medium, 90%; DMSO, 10%
Isotype
IgG2b; kappa light chain
Name of Depositor
Syntex (USA), Inc.
Deposited As
mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Allison AC, et al. Monoclonal antibodies for interleukin-1.beta. US Patent 4,935,343 dated Jun 19 1990

Kenney JS, et al. Monoclonal antibodies to human recombinant interleukin 1 (IL 1)beta: quantitation of IL 1 beta and inhibition of biological activity. J. Immunol. 138: 4236-4242, 1987. PubMed: 2438338

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