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J.gamma1

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價格:詢價

編號:B164867

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

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產品名稱

J.gamma1

商品貨號

B164867

Organism

Homo sapiens, human

Product Format

frozen

培養基

Morphology

lymphoblast

Culture Properties

clusters in suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease

acute T cell leukemia

Gender

male

Derivation

Wild-type Jurkat cells were mutagenized with the frameshifting mutagen ICR-191 and clones were isolated that failed to increase calcium in response to pervanadate. The selection procedure was repeated on the P98 clone to establish the J.gamma1 cell line.

Clinical Data

male

Comments

The J.gamm1 cell line is a phospholipase C-gamma1 (PLC-gamma1) deficient mutant of the E6-1 clone of Jurkat (ATCC TIB-152).

The J.gamma1.WT derivative (ATCC CRL-2679) stably expresses PLC-gamma1at levels comparable to the Jurkat cell line.

The J.gamma1 subline contains no detectable PLC-gamma1 protein. The lack of PLC-gamma1 expression in J.gamma1 cells causes profound defects in T cell receptor (TCR) calcium mobilization and nuclear factor of activated T-cells (NFAT) activation.

These transcriptional defects are reversed by transfection of P98 cells with a wild-type PLC-gamma1 expression vector.

Complete Growth Medium

The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.

Subculturing

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10⁵ viable cells/mL.

Maintain cell density between 1 X 10⁵ and 2 to 3 X 10⁶ viable cells/mL.

Cryopreservation

culture medium 95%; DMSO, 5%

Culture Conditions

Temperature: 37°C

STR Profile

Amelogenin: X

CSF1PO: 11,12

D13S317: 8,12

D16S539: 11

D5S818: 9

D7S820: 8,12

THO1: 6,9.3

TPOX: 8,10

vWA: 18,19

Name of Depositor

RT Abraham

Deposited As

human

References

Irvin BJ, et al. Pleiotropic contributions of phospholipase C-gamma1 (PLC-gamma1) to T-cell antigen receptor-mediated signaling reconstitution studies of a PLC-gamma1-deficient Jurkat T-cell line. Mol. Cell. Biol. 20: 9149-9161, 2000. PubMed: 11094067

B164868：J.gamma1.WT

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