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J5-1
J5-1
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B164876
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
J5-1
商品貨號(hào)
B164876
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphocyte
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The antibody reacts with endotoxins from Gram negative bacteria, and inhibits endotoxin induced mitogenesis of B cells.
The antibody is protective in mice against Pseudomonas aeruginosa burn infection.
Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation
Animals were immunized with purified E. coli J5 endotoxin core.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Escherichia coli J5 endotoxin core
Cellular Products
immunoglobulin; monoclonal antibody; against Escherichia coli J5 endotoxin core
Tumorigenic
Yes
Effects
Yes, forms ascites in pristane primed BALB/c mice
Comments
Animals were immunized with purified E. coli J5 endotoxin core.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with endotoxins from Gram negative bacteria, and inhibits endotoxin induced mitogenesis of B cells.
The antibody is protective in mice against Pseudomonas aeruginosa burn infection.
The epitope recognized by J5-1 appears to be distinct from that recognized by J5-2 (ATCC HB-8298).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation
RPMI 1640 medium with non-essential amino acids, 1 mM sodium pyruvate and 2 mM L-glutamine, 72.5%; fetal bovine serum, 20%; DMSO, 7.5%
Isotype
IgM
Name of Depositor
Centocor, Inc.
U.S. Patent Number
References

Pollack M, Hunter KW. Monoclonal antibodies reactive with endotoxin core. US Patent 5,057,598 dated Oct 15 1991

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