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J82
J82
規格:
價格:詢價
編號:B164880
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
J82
商品貨號
B164880
Organism
Homo sapiens, human
Tissue
urinary bladder
培養基
Product Format
frozen
Morphology
epithelial
Culture Properties
adherent
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
transitional cell carcinoma
Age
58 years
Gender
male
Ethnicity
Caucasian
Storage Conditions
liquid nitrogen vapor phase
Karyotype
The cell line is aneuploid human male (XY), with most chromosome counts in the triploid range. However, the chromosome count range is quite broad, extending from hyperdiploid to hexaploid. Normal chromosomes N11 and N20 are under-represented with respect to the other normal chromosomes: altered forms of these two chromosomes are prominent as marker chromosomes. Chromosome N13 tends towards over-representation. Five marker chromosomes are noted: 20q+, 11q+, 8p+, del(1)(q31), 5p+(HSR). One of these, 20q, is identical to marker chromosome M1 of C. O'Toole, et al., Br. J. Cancer 38: 64, 1978. The remainder are not as clearly related to the original marker chromosomes noted by those authors.
Clinical Data
male
58 years
Caucasian, Swedish
Antigen Expression
HLA A2, Aw32, B5, B12, Cw5; Blood Type A
Tumorigenic
Yes
Effects
Yes, in nude mice
Comments

Electron microscopic examination did not reveal desmosomes but varying amounts of rough endoplasmic reticulum and prominent microfilaments were observed.

Contains the ras (H-ras) oncogene.
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 10,12
D16S539: 11,12
D5S818: 12,13
D7S820: 9,11
THO1: 9.3
TPOX: 11,12
vWA: 17,18
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1-2
PGM1, 1
PGM3, 2
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