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JAA-F11
JAA-F11
規(guī)格:
價(jià)格:詢價(jià)
編號(hào):B164883
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
JAA-F11
商品貨號(hào)
B164883
Organism
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type
hybridoma: B lymphoblast
培養(yǎng)基
Product Format
frozen
Morphology
lymphoblast
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
Adenocarcinoma
Applications
The hybridoma cell line JAA-F11 was established in 1995.
The hybridoma produces an antibody (IgG3) that binds the saccharide antigen, Gal beta1-3GalNAc (T-antigen).
The antibody binds to breast adenocarcinoma tissue but not to normal tissues.
It may be useful in tumor studies, germinal center studies and T cell maturation studies.
The JAA-F11 cell line was formed by the fusion of P3X63Ag8.653 mouse myeloma cells with splenocytes from a BALB/c mouse inoculated with a synthetic Gal beta1-3GalNAc-BSA conjugate.
Storage Conditions
liquid nitrogen vapor phase
Derivation
The hybridoma cell line JAA-F11 was established in 1995.
Genes Expressed
immunoglobulin; monoclonal antibody; against the saccharide antigen, Gal beta1-3GalNAc (T antigen)
Cellular Products
immunoglobulin; monoclonal antibody; against the saccharide antigen, Gal beta1-3GalNAc (T antigen)
Comments
The hybridoma cell line JAA-F11 was established in 1995.
The hybridoma produces an antibody (IgG3) that binds the saccharide antigen, Gal beta1-3GalNAc (T-antigen).
The saccharide antigen, Gal beta1-3GalNAc is of biologic importance in many systems.
It is a tumor-associated carbohydrate antigen, a temporally expressed antigen in germinal center B cells and cortical T cells, a parasite-associated antigen, a spermatozoa vitality marker and an antigen on aged red blood cells.
The antibody binds to breast adenocarcinoma tissue but not to normal tissues.
It may be useful in tumor studies, germinal center studies and T cell maturation studies.
The JAA-F11 cell line was formed by the fusion of P3X63Ag8.653 mouse myeloma cells with splenocytes from a BALB/c mouse inoculated with a synthetic Gal beta1-3GalNAc-BSA conjugate.
Complete Growth Medium
Dulbecco's modified Eagle's Medium with 4 mM L-glutamine adjusted to contain 4.5 g/L glucose and 1.5 g/L sodium bicarbonate and supplemented with: 0.1 mM NEAA, 1.0% MEM Vitamins and 10% fetal bovine serum
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2-3 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 2 x 105 and 5 x 105 cells/mL.  Do not allow the cell concentration to exceed 5 x 105 cells/mL.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype
IgG3; kappa light chain
Name of Depositor
K Rittenhouse-Diakun
Deposited As
mouse (B cell); mouse (myeloma)
Year of Origin
1995
References

Rittenhouse-Diakun K, et al. Development and characterization of monoclonal antibody to T-antigen: (gal beta1-3GalNAc-alpha-O). Hybridoma 17: 165-173, 1998. PubMed: 9627057

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

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