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JAWSII

規(guī)格:

價(jià)格:詢價(jià)

編號(hào):B164885

品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產(chǎn)品名稱

JAWSII

商品貨號(hào)

B164885

Organism

Mus musculus, mouse

Tissue

bone marrow

培養(yǎng)基

Cell Type

immature dendritic cell; monocyte

Product Format

frozen

Morphology

monocyte

Culture Properties

mixed, adherent and suspension

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

C57BL/6

Storage Conditions

liquid nitrogen vapor phase

Disclosure

This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.

Complete Growth Medium

Alpha minimum essential medium with ribonucleosides, deoxyribonucleosides, 4 mM L-glutamine, 1 mM sodium pyruvate and 5 ng/ml murine GM-CSF, 80%; fetal bovine serum, 20%

Subculturing

Cultures can be maintained by transferring floating cells to a centrifuge tube.
Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA.
Pool cells and centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Note: This cell line grows very slowly.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: Once per week

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C

Name of Depositor

ZymoGenetics, Inc.

U.S. Patent Number

5,648,219

References

MacKay VL, Moore EE. Immortalized dendritic cells. US Patent 5,648,219 dated Jul 15 1997

Moore EE. Preparation of immortalized cells. US Patent 5,830,682 dated Nov 3 1998

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